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人抗乙型肝炎病毒核心抗體酶免試劑盒,(HBcAb)ELISA檢測試劑盒 ,英文名:HBcAb ELISA Kit ,英文縮寫:HBcAb ,常見試劑盒規格:96T/48T,產品別名:人抗乙型肝炎病毒核心抗體ELISA檢測試劑盒,HBcAb 檢測試劑盒, HBcAb 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。人抗乙型肝炎病毒核心抗體酶免試劑盒,(HBcAb)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重
纖維蛋白原α(FGα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibrinogen Alpha (FGa)48T/96TY09085Homo sapiens (Human)
絲裂原激活蛋白激酶8(MAPK8)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase 8 (MAPK8)48T/96TY09086Homo sapiens (Human)
155kDa核孔蛋白(NUP155)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleoporin 155kDa (NUP155)48T/96TY09087Mus musculus (Mouse)
四旋蛋白27(TSPAN27)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tetraspanin 27 (TSPAN27)48T/96TY09088Homo sapiens (Human)
胱天蛋白酶10(CASP10)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Caspase 10 (CASP10)48T/96TY09089Homo sapiens (Human)
硫氧還蛋白結合蛋白2(TBP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thioredoxin Binding Protein 2 (TBP2)48T/96TY09090Homo sapiens (Human)
發動蛋白2(DNM2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dynamin 2 (DNM2)48T/96TY09091Homo sapiens (Human)
磷脂爬行酶2(PLSCR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phospholipid Scramblase 2 (PLSCR2)48T/96TY09092Homo sapiens (Human)
狼瘡腦抗原(LBA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lupus Brain Antigen 1 (LBA1)48T/96TY09093Homo sapiens (Human)
VGF神經生長因子誘導蛋白(VGF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for VGF Nerve Growth Factor Inducible (VGF)48T/96TY09094Homo sapiens (Human)
雄激素誘導蛋白1(AIG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Androgen Induced Protein 1 (AIG1)48T/96TY09095Homo sapiens (Human)
214kDa核孔蛋白(NUP214)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleoporin 214kDa (NUP214)48T/96TY09096Homo sapiens (Human)
免疫抑制酸性蛋白(IAP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Immunosuppressive Acidic Protein (IAP)48T/96TY09097Homo sapiens (Human)
抗內因子抗體(AIFA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Anti-Intrinsic Factor Antibody (AIFA)48T/96TY09098Homo sapiens (Human)
腦啡肽(ENK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Enkephalin (ENK)48T/96TY09099Homo sapiens (Human)
低分子量激肽原(LMWK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Low Molecular Weight Kininogen (LMWK)48T/96TY09100Homo sapiens (Human)
205kDa核孔蛋白(NUP205)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleoporin 205kDa (NUP205)48T/96TY09101Homo sapiens (Human)
簇集蛋白(CLU)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Clusterin (CLU)48T/96TY09102Homo sapiens (Human)
抗原提呈關聯轉運蛋白(TAP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transporter Associated With Antigen Processing (TAP)48T/96TY09103Homo sapiens (Human)
188kDa核孔蛋白(NUP188)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleoporin 188kDa (NUP188)48T/96TY09104Homo sapiens (Human)
皮膚T-淋巴細胞關聯抗原(CTAGE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cutaneous T-Cell Lymphocyte Associated Antigen (CTAGE)48T/96TY09105Homo sapiens (Human)
葡萄糖轉運蛋白1(GLUT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glucose Transporter 1 (GLUT1)48T/96TY09106Homo sapiens (Human)
神經激肽B(NKB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neurokinin B (NKB)48T/96TY09107Homo sapiens (Human)
神經降壓素(NT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neurotensin (NT)48T/96TY09108Homo sapiens (Human)
接觸蛋白2(CNTN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Contactin 2 (CNTN2)48T/96TY09109Homo sapiens (Human)
腎上腺髓質素(ADM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adrenomedullin (ADM)48T/96TY09110Homo sapiens (Human)
絲裂原激活蛋白激酶14(MAPK14)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase 14 (MAPK14)48T/96TY09111Homo sapiens (Human)
生長激素誘導跨膜蛋白(GHITM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Hormone Inducible Transmembrane Protein (GHITM)48T/96TY09112Homo sapiens (Human)
接觸蛋白4(CNTN4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Contactin 4 (CNTN4)48T/96TY09113Homo sapiens (Human)
顆粒酶K(GZMK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Granzyme K (GZMK)48T/96TY09114Mus musculus (Mouse)
視網膜母細胞瘤蛋白1(RB1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Retinoblastoma Protein 1 (RB1)48T/96TY09115Homo sapiens (Human)
嗜鉻蛋白A(CHGA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chromogranin A (CHGA)48T/96TY09116Homo sapiens (Human)
毒蕈堿型膽堿受體M2(CHRM2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 2 (CHRM2)48T/96TY09117Homo sapiens (Human)
緩激肽(BK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Bradykinin (BK)48T/96TY09118Homo sapiens (Human)
纖維蛋白原β(FGβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibrinogen Beta (FGb)48T/96TY09119Homo sapiens (Human)
松弛肽(RLN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Relaxin (RLN)48T/96TY09120Homo sapiens (Human)
TGFβ誘導早期應答基因1(TIEG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for TGF Beta Inducible Early Response Gene 1 (TIEG1)48T/96TY09121Rattus norvegicus (Rat)
激素脫落酸(ABA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Abscisic Acid (ABA)48T/96TY09122General
胎盤堿性磷酸酶(ALPP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase, Placental (ALPP)48T/96TY09123Homo sapiens (Human)
接觸蛋白關聯蛋白樣蛋白4(CNTNAP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Contactin Associated Protein Like Protein 4 (CNTNAP4)48T/96TY09124Homo sapiens (Human)
糖抗原50(CA50)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carbohydrate Antigen 50 (CA50)48T/96TY09125Homo sapiens (Human)
突觸核蛋白α(SNCα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Synuclein Alpha (SNCa)48T/96TY09126Homo sapiens (Human)
神經細胞黏附分子(NCAM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neural Cell Adhesion Molecule (NCAM)48T/96TY09127Homo sapiens (Human)
抗補體1q抗體(Anti-C1q)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Anti-Complement 1q Antibody (Anti-C1q)48T/96TY09128Homo sapiens (Human)
胃泌素(GT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gastrin (GT)48T/96TY09129Homo sapiens (Human)
毒蕈堿型膽堿受體M4(CHRM4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 4 (CHRM4)48T/96TY09130Homo sapiens (Human)
MAX基因關聯蛋白A(MgA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for MAX Gene Associated Protein (MgA)48T/96TY09131Homo sapiens (Human)
細胞毒性T-淋巴細胞關聯抗原4(CTLA4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4)48T/96TY09132Homo sapiens (Human)
角蛋白18(KRT18)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Keratin 18 (KRT18)48T/96TY09133Homo sapiens (Human)
細胞外基質磷酸糖蛋白(MEPE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Matrix Extracellular Phosphoglycoprotein (MEPE)48T/96TY09134Homo sapiens (Human)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。